Darobactin B Stabilises a Lateral‐Closed Conformation of the BAM Complex in <i>E. coli</i> Cells

The β-barrel assembly machine (BAM) is essential for folding outer membrane proteins (OMPs) into the (OM) of Gram-negative bacteria. Structures BAM have been solved using X-ray crystallography and cryoEM, but conformation in highly crowded native OM remained unsolved. Using EPR spectroscopy intact E. coli cells, structure situ has now revealed Research Article (e202218783) by Sheena Radford, Christos Pliotas et al., both alone presence antibacterial, darobactin B. Image: Jeroen Claus (Phospho Biomedical Animation).